A method for the assay of phosphatidylinositol-specific phospholipase D activity in serum
β Scribed by Frank D. Raymond; Giuliana Fortunato; Donald W. Moss
- Book ID
- 115827761
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 772 KB
- Volume
- 215
- Category
- Article
- ISSN
- 0009-8981
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π SIMILAR VOLUMES
Phospholipase A 2 (PLA 2 ) is an enzyme that cleaves fatty acids from the sn-2 position of membrane phospholipids. Intracellular PLA 2 has been shown to be important for the generation of arachidonic acid, which leads to the synthesis of a wide range of eicosanoid paracrine hormones. Furthermore, se
We describe the preparation of phosphatidyl p-nitrophenol (PpNP) and its use as a substrate in an assay for the quantitative evaluation of Phospholipase D. The solution of the substrate in Tris HCl buffer, pH 8, and Triton X is mixed directly with an aliquot of fermentation broth and the absorbance