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A method for studying binding proteins, based upon differential dissociation of small ligand

โœ Scribed by Edwin Milgrom; Etienne-Emile Baulieu

Book ID
115748290
Publisher
Elsevier Science
Year
1969
Weight
271 KB
Volume
194
Category
Article
ISSN
0005-2795

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A novel spectroscopic titration method f
A novel spectroscopic titration method for determining the dissociation constant and stoichiometry of protein-ligand complex
โœ Zhi-Xin Wang; N. Ravi Kumar; D.K. Srivastava ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 579 KB

We offer a new titration protocol for determining the dissociation constant and binding stoichiometry of protein-ligand complex, detectable by spectroscopic methods. This approach neither is limited to the range of protein or ligand concentrations employed during titration experiment nor relies on p