𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A fluorescence-based assay for nanogram quantification of proteins using a protein binding ligand

✍ Scribed by H. Yamini Shrivastava; Balachandran Unni Nair

Book ID
105887987
Publisher
Springer
Year
2002
Tongue
English
Weight
224 KB
Volume
375
Category
Article
ISSN
1618-2650

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

A fluorescence-based detergent binding a
A fluorescence-based detergent binding assay for protein hydrophobicity
✍ Erwin London πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 586 KB

Protein hydrophobicity is often detected by binding of protein to micelles of a mild detergent. A new fluorescence method for detection of this binding is presented. The method is based on a long-range quenching of tryptophan fluorescence by energy transfer to a pyrene-labeled phospholipid probe inc

A protein-binding assay for phallotoxins
A protein-binding assay for phallotoxins using muscle actin
✍ A.J. SchΓ€fer; H. Faulstich πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 235 KB

The high affinity of phahotoxins to filamentous actin (K, = 3.6 x 1O-8 M) has been used to assay small amounts of phallotoxins. The limit of detection was as low as 160 ng of phallotoxins. This is 50 times lower than the limit of all other procedures known so far. The assay was successfully applied