✦ LIBER ✦

A Fluorescence-Based Protein Assay for Use with a Microplate Reader

✍ Scribed by A. Lorenzen; S.W. Kennedy

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 267 KB
Volume: 214
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1504

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A Microplate Reader-Based Nonisotopic Hi

A Microplate Reader-Based Nonisotopic Histone Deacetylase Activity Assay

✍ Birgit Heltweg; Manfred Jung 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 96 KB

Recent years have brought an enormous increase in knowledge concerning the involvement of histone deacetylase (HDAC) in gene regulation and the potential use of its inhibitors in transcription therapy. This also stimulates research toward new methods for the determination of HDAC activity and thus t

A Fluorescent Microplate Assay for Micro

A Fluorescent Microplate Assay for Microcystin-LR

✍ O.I. Fontal; M.R. Vieytes; J.M.V. Baptista de Sousa; M.C. Louzao; L.M. Botana 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 103 KB

A Fluorescent Microplate Assay for Diarr

A Fluorescent Microplate Assay for Diarrheic Shellfish Toxins

✍ M.R. Vieytes; O.I. Fontal; F. Leira; J.M.V.Baptista de Sousa; L.M. Botana 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 127 KB

Fluorescence Microplate-Based Assay for

Fluorescence Microplate-Based Assay for Tumor Necrosis Factor Activity Using SYTOX Green Stain

✍ Laurie J. Jones; Victoria L. Singer 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 112 KB

We have developed a simple, sensitive, fluorescence microplate-based assay for tumor necrosis factor (TNF) biological activity. The assay employs SYTOX Green nucleic acid stain to detect TNF-induced cell necrosis in actinomycin D sensitized cultured cell lines. SYTOX Green stain is a cationic unsymm

Use of a Fluorescence Microplate Reader

Use of a Fluorescence Microplate Reader for the Detection and Characterization of Metal-Assisted Peptide Hydrolysis

✍ Kathryn B. Grant; Sowmya Pattabhi 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 113 KB

Metal ions and complexes that hydrolyze peptides under nondenaturing conditions of temperature and pH hold great promise for use in protein structural studies. However, the extreme stability of the peptide amide bond has placed limits on the number of reagents available. In addition, the development

Development of a Green Fluorescent Prote

Development of a Green Fluorescent Protein Microplate Assay for the Screening of Chemopreventive Agents

✍ Ming Zhu; William E. Fahl 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 278 KB

Here we develop a rapid, cell-based, functional assay to screen and identify naturally occurring or synthetic chemicals with chemopreventive activity. We constructed a reporter gene that consists of the geneencoding green fluorescent protein (GFP) under the transcriptional control of the thymidine k