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A method for quantitation of protein in the presence of percoll

✍ Scribed by Masood N. Khan; Rahat J. Khan; Barry I. Posner

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
361 KB
Volume
117
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple assay for protein in the presence of Percoll which requires neither additional instrumentation nor manipulation of the sample is proposed. The method is based on monitoring the absorbance at 750 and 420 nm of color deveioped by protein plus Percoll with Folin-Ciocalteu reagent. Protein can be determined in a sample containing as much as 30% Percoll. The method does not require the knowledge of Percoll concentration (below 30%) in the sample. A large number of samples from a Percoll density gradient can be assayed very conveniently and with great precision.

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