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A method for purifying high quality and high yield plasmid DNA for metagenomic and deep sequencing approaches

✍ Scribed by Brown Kav, Aya; Benhar, Itai; Mizrahi, Itzhak

Book ID
121225521
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
854 KB
Volume
95
Category
Article
ISSN
0167-7012

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✍ Awdhesh Kalia; Ashok Rattan; Prem Chopra πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 143 KB

In this study, we report a modified procedure for extraction of high-quality genomic DNA that is rapid, simple, biologically nonhazardous, and generally applicable to pathogenic bacteria. Bacterial cells were pretreated with 70% ethanol prior to enzymatic digestion with lysozyme. Exposure of bacteri