A Simple Method for Isolating DNA of High Yield and Quality from Cotton (shape Gossypium hirsutum L.) Leaves
โ Scribed by Hugo R. Permingeat; Maria V. Romagnoli; Juliana I. Sesma; Ruben H. Vallejos
- Book ID
- 110250478
- Publisher
- Springer
- Year
- 1998
- Tongue
- English
- Weight
- 9 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0735-9640
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The isolation of biologically active RNA from cotton (Gossypium hirsutum L.) is difficult due to interference by high levels of endogenous phenolics, polysaccharides, and secondary metabolites. A modified hot borate procedure was developed to combat these cellular constituents during tissue homogeni
## Abstract **BACKGROUND:** A fast and easy method to isolate RNA from grape (__Vitis vinifera__) berries is proposed. **RESULTS:** The procedure uses high concentrations of cetyltrimethylammonium bromide (CTAB) and includes boric acid with the purpose of increasing the RNA extraction. Eppendorf t