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A Simple, High-Resolution Method for Establishing DNA Binding Affinity and Sequence Selectivity

✍ Scribed by Boger, Dale L.; Fink, Brian E.; Brunette, Steven R.; Tse, Winston C.; Hedrick, Michael P.


Book ID
115491853
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
871 KB
Volume
123
Category
Article
ISSN
0002-7863

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We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble