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A method for purification of bacterial R-type lipopolysaccharides (lipooligosaccharides)

✍ Scribed by Lihua Wu; Chao-Ming Tsai; Carl E. Frasch

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
782 KB
Volume
160
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new get fihration method was developed for purification of R-type Iipopolysaccharides (lipooligosaccharides) from some nonenteric gram-negative bacteria, including Neisseria meningitidis, Haemophilus influenzae, and Bordetella pertussis. These wild-type lipooligosaccharides are poorly extractable by the phenol-chloroform-ether extraction method of C. Galanos, 0. Luderitz, and 0. Westphal (( 1969) Eur. J. Biochem. 9, 245-249) and therefore a new procedure was developed for their isolation. The lipooligosaccharides (LOS) were first extracted by hot phenol-water, treated with RNase, then d&aggregated in deoxycholic acid, and purified by gel filtration on Sephadex G-75. By comparison the conventional hot phenol-water purification method using repeated ultracentrifugations yielded less LOS. The yield of LOS by gel filtration was 30 to 108% higher and the purity was better.

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