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A simple HPLC-MS method for the quantitative determination of the composition of bacterial medium chain-length polyhydroxyalkanoates

✍ Scribed by Andreas Grubelnik; Luzia Wiesli; Patrick Furrer; Daniel Rentsch; Roland Hany; Veronika R. Meyer

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
418 KB
Volume
31
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Bacterial poly(hydroxyalkanoates) (PHAs) vary in the composition of their monomeric units. Besides saturated side‐chains, unsaturated ones can also be found. The latter leads to unwanted by‐products (THF ester, secondary alcohols) during acidic cleavage of the polymer backbone in the conventional analytical assays. To prevent these problems, we developed a new method for the reductive depolymerization of medium chain‐length PHAs, leading to monomeric diols that can be separated and quantified by HPLC/MS. Reduction is performed at room temperature with lithium aluminum hydride within 5–15 min. The new method is faster and simpler than the previous ones and is quantitative. The results are consistent with the ones obtained by quantitative ^1^H NMR.

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