𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A method for high-frequency DNA-mediated transformation of plant protoplasts

✍ Scribed by C. Waldron; S. K. Malcolm; E. B. Murphy; J. L. Roberts

Book ID
112927105
Publisher
Springer
Year
1985
Tongue
English
Weight
230 KB
Volume
3
Category
Article
ISSN
0735-9640

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High frequency transformation of Bacillu
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA
✍ Chang, Shing ;Cohen, Stanley N. πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra