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In vitro transformation of plant protoplasts with Ti-plasmid DNA

✍ Scribed by Krens, F. A.; Molendijk, L.; Wullems, G. J.; Schilperoort, R. A.


Book ID
109725732
Publisher
Nature Publishing Group
Year
1982
Tongue
English
Weight
528 KB
Volume
296
Category
Article
ISSN
0028-0836

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Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the