Computer calculated isokinetic gradients provide superior uniform resolution for zone centrifugation of macromolecules and particles of known density (l-3). The technique is particularly useful in that it has the capacity to provide sedimentation coefficients without cumbersome routine computations
A method for controlled and accurate collection of sucrose density gradient fractions
โ Scribed by H.Curt Bubel; Barbara P. Riley
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 793 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The Multiskan spectrophotometric system and Coomassie brilliant blue G-250 protein-dye binding method have been used together to measure NaOH-solubilized protein in subcellular membrane fractions prepared from isolated rat adipose cells. Forty-eight samples can be read in duplicate within 1 min. Suc
Sonically disrupted nuclei from proliferating liver cells were fractionated in Cs2S0, equilibrium density gradients. Nuclear constituents were concentrated in three bands designated as light band (LB, 1.21 g/cm"), middle band (MB, 1.26 g/cm"), and heavy band (HB, 1.32 g/cm"). Analysis of protein and