Abstract
Carbonyl compounds (CC) play an important role in beverage aroma since they may affect flavor of wines, brandies, and beers, among others. For this reason, it is necessary to identify and quantify CC through adequate analytical techniques. This study is a proposal of both developing and optimization of a new analytical methodology that allows investigate C~1~βC~8~ CC in wines simultaneously by quantifying even those ones that are predominantly present in the adduct form hydroxylalkylsulfonic acids (HASA). The HASA dissociation is undertaken by specific alkaline media (pH 11). The developed methodology employed the LC with UV/VIS detection (Ξ» = 365 nm) technique under gradient elution in the way to reach both freeβCC and boundβCC quantification. Results showed that binary gradient system using eluent A (MeOH/ACN/H~2~O 74.5:0.5:25% v/v/v) and eluent B (MeOH) reached the best separation condition of both lower and higher molecular mass CC. This proposed method allowed simultaneous quantification of formaldehyde, acetaldehyde, propanone, furfuraldehyde, butyraldehyde, benzaldehyde, hexanaldehyde, 2βethylβhexanaldehyde, Eβpentβ2βenβ1βal, and cyclohexanone β all of them were found in white wine (Moscato Canelli) and red wine (Shiraz) produced in the SΓ£o Francisco Valley, in the Northeastern Region of Brazil β although this optimized method may probably be suitable for quantification of propionaldehyde, isobutyraldehyde, heptanaldehyde, octanaldehyde, benzaldehyde, and Eβhexβ2βenβ1βal as well. We could not prove if this method is also able to determine the latter CC group since we have not found these substances present in detectable levels in our real samples considered in this study.