A linear standard curve for the Folin Lowry determination of protein
โ Scribed by Clyde E. Stauffer
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 186 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The protein method of Lowry, Rosebrough, Farr and Randall was modified to give a linear standard curve of absorbance versus pg of bovine serum albumin at 650 and 750 nm wavelengths (1 cm optical path) over the range up to 50 pg protein per ml and an absorbance of 1.1. This was achieved mainly by usi
Certain reagents utilized in the formation of polyacrylamide gels are shown to interfere in the Lowry assay for protein. Acrylamide (3-30%) and potassium ferrocyanide (O.OOlS-0.0105%) produced a linear response in color formation. Both compounds are capable of reducing the phenol reagent in the abse