A linear Lowry-Folin assay for both water-soluble and sodium dodecyl sulfate-solubilized proteins
✍ Scribed by Helen H. Hess; Marjorie B. Lees; Julia E. Derr
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 366 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The protein method of Lowry, Rosebrough, Farr and Randall was modified to give a linear standard curve of absorbance versus pg of bovine serum albumin at 650 and 750 nm wavelengths (1 cm optical path) over the range up to 50 pg protein per ml and an absorbance of 1.1. This was achieved mainly by using a high concentration of Folin-phenol reagent, added rapidly in a volume that was large relative to the final volume. Sodium dodecyl sulfate (SDS) incorporated in the test did not change the albumin standard curve, and linear results were obtained with water-insoluble membrane proteins (myelin proteolipid and rhodopsin) solubilized by SDS.