✦ LIBER ✦

A ligand-receptor binding assay by receptor immobilization

✍ Scribed by Masao Takami; Izumi Kasuya; Hajime Tsunoo

Book ID: 102630186
Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 398 KB
Volume: 170
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90113-3

No coin nor oath required. For personal study only.

✦ Synopsis

Using transferrin-transferrin receptor binding as a model of ligand-receptor binding, we have developed a new and simple binding assay for the solubilized receptor. Solubilized membrane proteins containing transferrin receptor were immobilized by covalent binding to beads having chemical reactive epoxide groups, and then 125I-labeled transferrin was added to the beads. Dose-dependent, ligand-specific, and saturable binding of 125I-labeled transferrin to the immobilized membrane proteins were demonstrated and a Scatchard analysis derived affinity of Kd = 1.8 X 10(-9) M was obtained. These results indicate that the immobilization of receptors onto beads may be useful in a simple binding assay of the solubilized receptor.

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