๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Nonradioactive ligand binding assay for epidermal growth factor receptor

โœ Scribed by Ivan C. King; Joseph J. Catino

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
367 KB
Volume
188
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A rapid and sensitive nonradioactive ligand binding assay for identifying the agonists and antagonists of epidermal growth factor receptor is described. The assay is easy to perform, has a within-assay error of less than 15%, and does not use radioactive substances. Nonspecific binding is less than 10% and the assay is sensitive enough to detect epidermal growth factor (EGF) antagonists at as little as 10 nM, if the antagonist has the same affinity as EGF. The assay is carried out in 96-well plates and is particularly suitable for large-scale screening. This report includes description of the methods that we used to assess the validity of the assay system, and the results of that assessment.

๐Ÿ“œ SIMILAR VOLUMES

Ratiometric Assay of Epidermal Growth Fa
Ratiometric Assay of Epidermal Growth Factor Receptor Tyrosine Kinase Activation
โœ Kevin Schooler; H.Steven Wiley ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 148 KB

Activation of cells is frequently followed by tyrosine phosphorylation of proteins. To quantify this process, we developed a ratiometric enzyme-linked immunosorbent assay (ELISA) using epidermal growth factor receptors (EGFR) as a model. Microtiter dishes were coated with anti-EGFR monoclonal antibo

The epidermal growth factor receptor lig
The epidermal growth factor receptor ligands at a glance
โœ Marlon R. Schneider; Eckhard Wolf ๐Ÿ“‚ Article ๐Ÿ“… 2009 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 424 KB

## Abstract The epidermal growth factor receptor (EGFR) regulates key processes of cell biology, including proliferation, survival, and differentiation during development, tissue homeostasis, and tumorigenesis. Canonical EGFR activation involves the binding of seven peptide growth factors. These li

Europium-Labeled Epidermal Growth Factor
Europium-Labeled Epidermal Growth Factor and Neurotensin: Novel Probes for Receptor-Binding Studies
โœ Ohad Mazor; Marc Hillairet de Boisferon; Alain Lombet; Anne Gruaz-Guyon; Batya G ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 81 KB

We investigated the possibility of labeling two biologically active peptides, epidermal growth factor (EGF) and neurotensin (NT), with europium (Eu)-diethylenetriaminepentaacetic acid. More specifically, we tested them as probes in studying receptor binding using time-resolved fluorescence of Eu 3ุ‰

Comparison of radioligand assay and immu
Comparison of radioligand assay and immunostaining for epidermal growth factor receptor in human breast cancer
โœ D. J. Hastrich; J. M. Dunn; S. Nicholson; P. Newcomb; Professor J. R. Farndon ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 394 KB ๐Ÿ‘ 2 views

## Abstract Epidermal growth factor (EGF) receptor status is a useful prognostic indicator in women with breast cancer. Lack of standardization and correlation of methodology for the detection of EGF receptor has hampered its further evaluation. EGF receptor status was ascertained by immunohistoche

Heparin-binding epidermal growth factor-
Heparin-binding epidermal growth factor-like growth factor isoforms and epidermal growth factor receptor/ErbB1 expression in bladder cancer and their relation to clinical outcome
โœ Christopher Kramer; Kai Klasmeyer; Hans Bojar; Wolfgang A. Schulz; Rolf Ackerman ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 313 KB ๐Ÿ‘ 1 views

## Abstract ## BACKGROUND. Cleavage of membraneโ€anchored heparinโ€binding epidermal growth factorโ€like growth factor (proHBโ€EGF) yields a soluble HBโ€EGF isoform (sHBโ€EGF), which is an activating epidermal growth factor receptor (EGFR) ligand and a Cโ€terminal fragment HBโ€EGFโ€C acting directly in the