β Scribed by Dr. Hanspeter Saluz, Dr. Jean-Pierre Jost (auth.)
No coin nor oath required. For personal study only.
Front Matter....Pages 1-8
Introduction....Pages 9-13
Theoretical Background....Pages 15-22
Front Matter....Pages 23-23
Isolation of Genomic DNA....Pages 24-32
Restriction Digest of Genomic DNA....Pages 33-40
Chemical Sequencing Reactions on Restricted DNA....Pages 41-50
Separation of Reaction Products on a Sequencing Gel....Pages 51-58
Electrotransfer to Nylon Membranes....Pages 59-65
Immobilization of DNA on Nylon Membranes....Pages 66-72
Prehybridization and Hybridization of Immobilized DNA with Labeled Single-Stranded DNA Probes....Pages 73-84
Processing of the Hybridized Filters....Pages 85-89
Autoradiography and Photography....Pages 90-98
Cloning of DNA Probe in M13....Pages 99-105
Large-Scale Preparation of Cloned DNA in M13....Pages 106-110
Synthesis of Oligonucleotide Primers and Single-Stranded Labeled Probes....Pages 111-117
Purification of Labeled Single-Stranded Probes....Pages 118-124
Front Matter....Pages 125-126
Trouble-Shooting Guide....Pages 125-149
Appendix....Pages 151-156
Bibliography....Pages 157-160
Back Matter....Pages 161-163
Science, general
π SIMILAR VOLUMES
Univ. of Manchester, U.K. Practical, strategic guide to the process of DNA sequencing. Covers planning the approach, data acquisition, and extracting findings from the data. Softcover.
This volume provides a comprehensive description of the principles and methods used in DNA sequencing. Following a detailed introduction the chapters are: DNA sequencing; Chain terminator sequencing; Primed synthesis methods applied to DNA fragments cloned into phage M13; DNA sequencing by the Maxam
<p>Genetics mapping, physical mapping and DNA sequencing are the three key components of the human and other genome projects. Statistics, mathematics and computing play important roles in all three, as well as in the uses to which the mapping and sequencing data are put. This volume edited by key re
Genetics mapping, physical mapping and DNA sequencing are the three key components of the human and other genome projects. Statistics, mathematics and computing play important roles in all three, as well as in the uses to which the mapping and sequencing data are put. This volume edited by key resea