Univ. of Manchester, U.K. Practical, strategic guide to the process of DNA sequencing. Covers planning the approach, data acquisition, and extracting findings from the data. Softcover.
DNA Sequencing
โ Scribed by T.S. Work and R.H. Burdon (Eds.)
- Publisher
- Elsevier, Academic Press
- Year
- 1983
- Tongue
- English
- Leaves
- 397
- Series
- Laboratory Techniques in Biochemistry and Molecular Biology 10
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
This volume provides a comprehensive description of the principles and methods used in DNA sequencing. Following a detailed introduction the chapters are: DNA sequencing; Chain terminator sequencing; Primed synthesis methods applied to DNA fragments cloned into phage M13; DNA sequencing by the Maxam-Gilbert chemical procedure; Computer methods for DNA sequencers; Appendices including contractions and special terms, cloning vectors, commercially available restriction endonucleases, and autoradiography.
โฆ Table of Contents
Content:
Edited by
Pages ii-iii
Copyright page
Page iv
Acknowledgements
Page v
Chapter 1 Introduction
Pages 1-29
Chapter 2 DNA sequencing
Pages 30-71
Chapter 3 Chain terminator sequencing
Pages 72-120
Chapter 4 Primed synthesis methods applied to DNA fragments cloned into phage M13
Pages 121-229
Chapter 5 DNA sequencing by the Maxam-Gilbert chemical procedure
Pages 230-285
Update: Update to chapter 4
Pages 286-310
Chapter 6 Computer methods for DNA sequencers
Pages 311-348
Appendix to chapter 6
Pages 349-368
References
Pages 369-373
Subject Index
Pages 375-384
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