The amendment of the interpretation of recently published sizeโexclusion chromatography (SEC) data for Aโkinase anchoring protein (AKAP12) on SephacrylโS400 has led to an increase in the estimated size of the supermolecular state from 840 to at least 6000โkDa. Although sizeโexclusion chromatography
A-Kinase Anchoring Protein Targeting of Protein Kinase A and Regulation of HERG Channels
โ Scribed by Yan Li; Jakub Sroubek; Yamini Krishnan; Thomas V. McDonald
- Book ID
- 105926678
- Publisher
- Springer
- Year
- 2008
- Tongue
- English
- Weight
- 547 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0022-2631
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We report a simple method that permits simultaneous detection of multiple protein kinase activities using postnuclear supernatant of \(\mathbf{v}-8\) src transformed NIH3T3 cells. A supernatant is incubated with activators of protein kinases and \(\left[\gamma-{ }^{32} \mathbf{P}\right] \mathrm{ATP}
## Abstract Protein scaffold complexes are a key mechanism by which a common signaling pathway can serve many different functions. Sequestering a signaling enzyme to a specific subcellular environment not only ensures that the enzyme is near its relevant targets, but also segregates this activity t