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A hypotetraploid human T lymphoid cell line established by cell fusion

✍ Scribed by Kazuhiro Ishii; Junji Yodoi; Masao Hanaoka; Jun-Ichi Furuyama

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
605 KB
Volume
94
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

A human T lymphoid cell line was established by cell hybridization technique from peripheral blood leucocytes of a patient with Sezary syndrome. The cells beared the surface antigens of human T lymphocyte specificity as demonstrated by immune cytolysis tests, but did not form E rosettes with sheep red blood cells. Isozyme patterns of enzymes in this line such as lactate dehydrogenase, glucose 6-phosphate dehydrogenase and esterase were of human type. The line had 79 chromosomes in modal number. This case supports the proposal that the production of tetraploids is favourable for establishment of cell lines.

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