Supernatants of a mclnnorna cell line were chromatographed on five affinity supports to ev;ilu;itr and compare purification protocols for isolation of tissue-type plasminogen activator (t-Pa). t-PA was adsorbed qunntitittively from cell culture supernatants by lysine-Sepharose, concanavulin -A-Sepha
โฆ LIBER โฆ
High frequency of plasminogen activator secretion by malignant human lymphoid cell lines of T-cell type origin
โ Scribed by Dr. Anne-Marie Venuat; Claudine Soria; Jeannette Soria; Patricia Krief; Manouchehr Mirshahi; Lu He; Annick Thomaidis; Anne Houllier; Martine Billard; Claude Boucheix
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 791 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0008-543X
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L Y I 17018 is a non-steroid anti-estrogen which exhibits about 100 times higher affinity for estrogen receptor than tamoxifen, another anti-estrogen. The cell line ES-I , which was isolated from human breast cancer MCF-7 cells, was highly sensitive to the cytocidal action of estradiol. Growth of ES