Purification of human tissue-type plasminogen activator (t-PA) from melanoma cell culture medium by different affinity chromatographic methods
✍ Scribed by B. E. Fischer; U. Zacharias; D. Wernicke; H. Will
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 358 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0138-4988
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✦ Synopsis
Supernatants of a mclnnorna cell line were chromatographed on five affinity supports to ev;ilu;itr and compare purification protocols for isolation of tissue-type plasminogen activator (t-Pa). t-PA was adsorbed qunntitittively from cell culture supernatants by lysine-Sepharose, concanavulin -A-Sepharose, zinc-chelate-Sepharose and monoclonal antibody-Sepharose. The major p:rrt of t-PA wits effectively adsorbed also by heparin-Sepharose. About 20 per cent of t-PA activity piesent in the culture medium however was not bound to the latter support, which indicates a heterogeneity of melanoma t-PA. The heterogeneity is not due to carbohydrate variants I and I1 since both vnriants were adsorbed and eluted from all the affinity supports invcstignted. Highly enriched t-PA preparations were obtained by sequential chromatography on two supports. t-PA isolated by monoclonal antibody affinity chromatography and subsequently purified by gel filtration was of highest purity. Other useful combinations were chromatography on concanavalin A-Sephnrose or lysine-Sepharose followed by chromatography on heparin-Sepharose, or zinc-chelate-Seph;irose followed hy chromatography on lysine-Sepharose.