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Establishment of a human hemangiosarcoma cell line (ISO-HAS)

โœ Scribed by Mikio Masuzawa; Takao Fujimura; Yuhko Hamada; Yuhsuke Fujita; Hisamichi Hara; Shigeo Nishiyama; Kensei Katsuoka; Hidekazu Tamauchi; Yoshinori Sakurai

Publisher
John Wiley and Sons
Year
1999
Tongue
French
Weight
75 KB
Volume
81
Category
Article
ISSN
0020-7136

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โœฆ Synopsis

A cell line (ISO-HAS) has been established from tumor tissue of a human hemangiosarcoma arising on the scalp by the use of conditioned medium from a murine-phenotypic angiosarcoma cell line (ISOS-1). Cells have been cultured for more than 2 years with up to 100 passages. The cells retained endothelial-cell properties, such as a characteristic cobblestone appearance at confluency, contact-inhibited growth, active uptake of acetylated low-density lipoprotein labeled with 1,1-dioctadecyl 1,3,3,3,3-tetramethyl-indocarbocyanine perchlorate (Dil-Ac-LDL) and CD31 expression. However, they were weakly positive for von-Willebrand-factor (vWf) antigen and for binding of Ulex europaeus agglutinin-I (UEA-1) lectin, and lacked tube-formation activity. These findings indicate that ISO-HAS is a poorly differentiated endothelial cell line. ISO-HAS cells showed accumulation of p53 protein in the nuclei, and a new-typed p53-gene point mutation was found in exon 7 at codon 240. When inoculated s.c. into severe-combined-immunodeficiency (SCID) mice, the cells showed solid-tumor growth that caused death. These properties suggest that ISO-HAS is a malignant endothelial cell line with high tumorigenicity.

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