A histochemical method for demonstration of 2′ ,3′ -cyclic 3′ -phosphohydrolase activity
✍ Scribed by Zaprianova, Emilia; Dikov, Angel; Christova, Maria; Kadiisky, Dimitar
- Book ID
- 122674349
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- German
- Weight
- 917 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0065-1281
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## Abstract Activity of the myelin marker enzyme 2′,3′‐cyclic nucleotide 3′‐phosphohydrolase (CNP) was assayed in cerebrospinal fluid samples obtained from patients with multiple sclerosis (MS) and other neurological diseases. The enzyme activity was found to be elevated in acute cases of MS and re
A new continuous spectrophotometric method for determining 2',3'-cyclic nucleotide 3'-phosphohydrolase (EC 3.1.4.37) is described. The assay method involves monitoring the decrease in pH which accompanies the hydrolysis of 2',3'-cyclic AMP. The reaction is performed in the presence of phenol red and