A highly sensitive reverse transcription polymerase chain reaction method for yellow fever virus detection
✍ Scribed by J. Méndez; C. Mendez; C. Domingo; G. Rey; A. Tenorio
- Book ID
- 118451740
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 75 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1201-9712
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A novel system for the detection of polymerase chain reaction (PCR) products has been developed. The system is based on a PCR in which one of the primers is biotinylated and digoxigenin-11-dUTP is incorporated during elongation. Biotinylated PCR products are captured on streptavidin-coated solid sup
## Abstract A protocol was developed for a highly sensitive detection of viral RNA in blood specimens by reverse transcription coupled with a nested poly‐merase chain reaction. Using Japanese encephalitis virus (JEV) as a model, the optimized reverse transcription‐polymerase chain reaction (ORTPCR)