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A high-throughput cell-based reporter gene system for measurement of CYP1A1 induction

✍ Scribed by Xiaoming Cui; Jairam Palamanda; Laura Norton; Ann Thomas; Yau Yi Lau; Ronald E. White; K.-C. Cheng


Book ID
114343105
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
336 KB
Volume
47
Category
Article
ISSN
1056-8719

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The properties of M-hirudin as a new reporter gene system were examined using rabbit reticulocyte lysate for cell-free protein expression. In contrast to the luciferase gene, in vitro translation of M-hirudin is highly robust against changes in concentrations of K Ψ‰ (and Rb Ψ‰ ). In addition, M-hirud