𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Validation for high-throughput screening of a VanRS-based reporter gene assay for bacterial cell wall inhibitors

✍ Scribed by G. De Pascale; C. Grigoriadou; D. Losi; I. Ciciliato; M. Sosio; S. Donadio


Book ID
108838553
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
543 KB
Volume
103
Category
Article
ISSN
1364-5072

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


A Chimeric Reporter Gene Allowing for Cl
✍ Knut Kotarsky; Christer Owman; BjΓΆrn Olde πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 114 KB

Efficient screening of ligands interacting with Gprotein-coupled receptors is central for modern drug development. Here, we describe an optimized reporter vector primarily intended for use in reporter cell lines expressing such receptors. The construct consists of a synthetic enhancer containing 9Ψ‹

A Reporter Gene Assay for High-Throughpu
✍ Yves Durocher; Sylvie Perret; Eric Thibaudeau; Marie-Helene Gaumond; Amine Kamen πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 184 KB

We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclear antigen) (designated 293E) cells. The ability to grow these cells in suspension, in conjunction with the us