A high-performance liquid chromatographic procedure for the purification of mouse monoclonal antibodies
β Scribed by Jeffrey R. Deschamps; James E.K. Hildreth; Dwight Derr; J.Thomas August
- Book ID
- 107712189
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 373 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0003-2697
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Protein A, a bacterial cell wall protein found in Staphylococcus aureus, has been widely used for the analysis of immunoglobulins. By attaching protein A to a microparticulate silica support, a rapid and efficient chromatographic sorbent has been created for the separation of monoclonal antibodies.
Thiophilic adsorption chromatography, developed originally by Porath and colleagues (1985, FEBS Lett. 185, 306-310) for conventional chromatographic techniques, was transformed to the HPLC mode upon preparation of new and improved sulfur-containing silica beads. The new thiophilic adsorbent is of hi