We describe a mitochondrial suppressor mutation, which restores respiratory competence to the nuclear pet--mutant MK2. This mutant lacks the message of the mitochondrial cob-gene and instead accumulates a partially spliced pre-mRNA which ,is not translated. Complete processing and translation of the
✦ LIBER ✦
A high copy number of yeast γ-glutamylcysteine synthetase suppresses a nuclear mutation affecting mitochondrial translation
✍ Scribed by Thomas Lisowsky
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 752 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0172-8083
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Agene bank of a yeast wild type DNA in the high copy number vector YEpl3 was screened for recombinant plasmids which suppress the mitochondrial RNA splice defect exerted by mutant M1301, a -1 bp deletion in the first intron of the mitochondrial COB gone (bI1). A total of 17 recombinant plasmids with