Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number
✍ Scribed by Hans Koff; Cornelia Schmidt; Gerlinde Wiesenberger; Carlo Schmelzer
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 637 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0172-8083
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✦ Synopsis
Agene bank of a yeast wild type DNA in the high copy number vector YEpl3 was screened for recombinant plasmids which suppress the mitochondrial RNA splice defect exerted by mutant M1301, a -1 bp deletion in the first intron of the mitochondrial COB gone (bI1). A total of 17 recombinant plasmids with similar suppressor activity were found. Restriction mapping and cross-hybridization of the inserts revealed that these 17 plasmids contain three different inserts, all lacking any extended sequence homology. Each of the inserts, when present in high copy number, has a similar suppressor activity: high in the presence of mutation 311301 in bI1, a group II intron, and low but significant with the presence of few mutants in bI2 and bI3 of the COB gene, both of which are group I introns.