𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Heteronuclear Direct-Detection NMR Spectroscopy Experiment for Protein-Backbone Assignment

✍ Scribed by Ivano Bertini; Luminita Duma; Isabella C. Felli; Michael Fey; Claudio Luchinat; Roberta Pierattelli; Paul R. Vasos

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
112 KB
Volume
43
Category
Article
ISSN
0044-8249

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

A Sequential HNCA NMR Pulse Sequence for
A Sequential HNCA NMR Pulse Sequence for Protein Backbone Assignment
✍ Axel Meissner; Ole Winneche SΓΈrensen πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 90 KB

The conventional HNCA pulse sequence suffers from the ambiguity that it cannot distinguish inter-and intraresidue correlations because the one-bond and two-bond J(NC Ξ± ) coupling constants are of similar magnitude. This paper presents a novel pulse sequence, sequential HNCA, that leads to a spectrum

QQ-HSQC: a quick, quantitative heteronuc
QQ-HSQC: a quick, quantitative heteronuclear correlation experiment for NMR spectroscopy
✍ Daniel J. Peterson; Nikolaus M. Loening πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 167 KB

## Abstract Quantitative heteronuclear single quantum coherence (Q‐HSQC) is a variant of the HSQC experiment that provides quantitative peak areas. This is accomplished by combining signals acquired using four different INEPT delays. Consequently, the experiment requires four times as many scans as

Heteronuclear X-Filter1H PFG Double-Quan
Heteronuclear X-Filter1H PFG Double-Quantum Experiments for the Proton Resonance Assignment of a Ligand Bound to a Protein
✍ Claudio Dalvit; Paul Ramage; Ulrich Hommel πŸ“‚ Article πŸ“… 1998 πŸ› Elsevier Science 🌐 English βš– 161 KB

A novel X-filter experiment based on 1 H PFG DQ spectroscopy selection gradients which achieve excellent 13 C-bound prois described. Excellent suppression of proton bound to 13 C and 15 N ton suppression. The pulse sequences for different variants is achieved. Successful application of the method to