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Heteronuclear X-Filter1H PFG Double-Quantum Experiments for the Proton Resonance Assignment of a Ligand Bound to a Protein

✍ Scribed by Claudio Dalvit; Paul Ramage; Ulrich Hommel


Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
161 KB
Volume
131
Category
Article
ISSN
1090-7807

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✦ Synopsis


A novel X-filter experiment based on 1 H PFG DQ spectroscopy selection gradients which achieve excellent 13 C-bound prois described. Excellent suppression of proton bound to 13 C and 15 N ton suppression. The pulse sequences for different variants is achieved. Successful application of the method to a proteinof this experiment are shown in Fig. . The schemes in ligand complex is demonstrated. ᭧ 1998 Academic Press Figs. 1a and 1b are the 13 C-filter 2D double-quantum (DQ)