A Heteronuclear Direct-Detection NMR Spectroscopy Experiment for Protein-Backbone Assignment
β Scribed by Ivano Bertini; Luminita Duma; Isabella C. Felli; Michael Fey; Claudio Luchinat; Roberta Pierattelli; Paul R. Vasos
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 111 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0044-8249
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The conventional HNCA pulse sequence suffers from the ambiguity that it cannot distinguish inter-and intraresidue correlations because the one-bond and two-bond J(NC Ξ± ) coupling constants are of similar magnitude. This paper presents a novel pulse sequence, sequential HNCA, that leads to a spectrum
## Abstract Quantitative heteronuclear single quantum coherence (QβHSQC) is a variant of the HSQC experiment that provides quantitative peak areas. This is accomplished by combining signals acquired using four different INEPT delays. Consequently, the experiment requires four times as many scans as
A novel X-filter experiment based on 1 H PFG DQ spectroscopy selection gradients which achieve excellent 13 C-bound prois described. Excellent suppression of proton bound to 13 C and 15 N ton suppression. The pulse sequences for different variants is achieved. Successful application of the method to