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A genetic analysis system of Burkholderia cepacia: construction of mobilizable transposons and a cloning vector

✍ Scribed by Mitsuko Abe; Masataka Tsuda; Mitsuaki Kimoto; Sachiye Inouye; Atsushi Nakazawa; Teruko Nakazawa

Book ID
116133532
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
367 KB
Volume
174
Category
Article
ISSN
0378-1119

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We have constructed a cosmid derivative of the low copy-number broad host-range cloning vector pRK290 (Ditta et al., 1980) by inserting a 1.6kb Bg/II fragment containing X cos into the unique BglII site in pRK290. The new vector, pLAFR1, is 21.6 kb long, confers tetracycline resistance, contains a u