We have constructed a cosmid derivative of the low copy-number broad host-range cloning vector pRK290 (Ditta et al., 1980) by inserting a 1.6kb Bg/II fragment containing X cos into the unique BglII site in pRK290. The new vector, pLAFR1, is 21.6 kb long, confers tetracycline resistance, contains a u
โฆ LIBER โฆ
Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants
โ Scribed by Friedman, Alan M.; Long, Sharon R.; Brown, Susan E.; Buikema, William J.; Ausubel, Frederick M.
- Book ID
- 121295003
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 664 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0378-1119
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