๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants

โœ Scribed by Friedman, Alan M.; Long, Sharon R.; Brown, Susan E.; Buikema, William J.; Ausubel, Frederick M.


Book ID
121295003
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
664 KB
Volume
18
Category
Article
ISSN
0378-1119

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Construction of a broad host range cosmi
โœ Friedman, Alan M.; Long, Sharon R.; Brown, Susan E.; Buikema, William J.; Ausube ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 664 KB

We have constructed a cosmid derivative of the low copy-number broad host-range cloning vector pRK290 (Ditta et al., 1980) by inserting a 1.6kb Bg/II fragment containing X cos into the unique BglII site in pRK290. The new vector, pLAFR1, is 21.6 kb long, confers tetracycline resistance, contains a u

A multipurpose broad host range cloning
โœ Liu, Yu-Ning ;Tang, Ji-Liang ;Clarke, Belinda R. ;Dow, J. Maxwell ;Daniels, Mich ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 937 KB

A multipurpose broad host range plasmid, pIJ3200, was constructed by inserting the polylinker-containing 445 bp PvuII fragment of Bluescript M13 into the EcoRI site of the cosmid pLAFR1. Using this vector a protease gene of Xanthomonas campestris pathovar campestris, previously cloned in the recombi