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Construction of a broad host range vector for the regulated expression of cloned genes in a range of gram-negative bacteria

✍ Scribed by N. Mermod; K. N. Timmis


Publisher
Springer
Year
1986
Tongue
English
Weight
141 KB
Volume
42
Category
Article
ISSN
1420-682X

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A multipurpose broad host range cloning
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A multipurpose broad host range plasmid, pIJ3200, was constructed by inserting the polylinker-containing 445 bp PvuII fragment of Bluescript M13 into the EcoRI site of the cosmid pLAFR1. Using this vector a protease gene of Xanthomonas campestris pathovar campestris, previously cloned in the recombi