A fluorogenic assay for transketolase from Saccharomyces cerevisiae
✍ Scribed by Aurélie Sevestre; Virgil Hélaine; Ghislain Guyot; Christine Martin; Laurence Hecquet
- Book ID
- 104252727
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- French
- Weight
- 252 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0040-4039
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📜 SIMILAR VOLUMES
Electrophoretically homogeneous phosphoenolpyruvate carboxykinase (EC 4.1.1.49) from Saccharomyces cerevisiae was obtained in high yields by means of a two-step purification procedure consisting of ion-exchange chromatography and alhnity chromatography on adenosine 5'-monophosphate-Sepharose 4B. In
A simple and rapid HPLC assay is reported for the measurement and kinetic study of transketolase. The assay consists of monitoring the rate of conversion of j'$ hydroxypyruvate and glycolaldehyde to L-erythrulose. The assay is also useful in monitoring transketolase catalysed biotransformations for