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Half-of-the-sites reactivity of transketolase from Saccharomyces cerevisiae

✍ Scribed by Irina Sevostyanova; Olga Solovjeva; Vitaly Selivanov; German Kochetov


Book ID
116297967
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
239 KB
Volume
379
Category
Article
ISSN
0006-291X

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The decay kinetics of mRNA was studied in a yeast temperature-sensitive mutant, ts136, which is defective in cytoplasmic RNA production at 37 degree C. The disappearance of the synthetic capacity of mRNA was determined by withdrawing equal volumes of ts136 cell culture and pulse-labelling with [35S]