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A Flow Cytometric Assay for Lysosomal Glucocerebrosidase

✍ Scribed by Helmuth H.G. van Es; Marlon Veldwijk; Menzo Havenga; Dinko Valerio


Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
98 KB
Volume
247
Category
Article
ISSN
0003-2697

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## Abstract A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycin‐D for common binding sites on DNA coated 10 ΞΌm diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the pre