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A flow cytometric assay for intracellular nonprotein thiols using mercury orange

โœ Scribed by Jose Enrique O'Connor; Dr. Bruce F. Kimler; Michael C. Morgan; Kathryn J. Tempas

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
380 KB
Volume
9
Category
Article
ISSN
0196-4763

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A flow cytometric method for measurement
A flow cytometric method for measurement of intracellular chloride concentration in lymphocytes using the halide-specific probe 6-methoxy-N-(3-sulfopropyl) quinolinium (SPQ)
โœ Barbara Pilas; Gary Durack ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 88 KB ๐Ÿ‘ 2 views

A flow cytometry method using the halide-specific fluorescent dye, 6-methoxy-N-(3-sulfopropyl) quinolinium (SPQ), has been developed to measure intracellular chloride concentration in single cells. Collisions with chloride quench the fluorescence of SPQ, making it possible to relate the measured flu