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A filtration method for rapid preparation of conjugates for immunoassay

✍ Scribed by Tarun K. Dhar; Subham Dasgupta; Dipika Ray; Meghna Banerjee

Book ID
119298668
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
429 KB
Volume
385
Category
Article
ISSN
0022-1759

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Two methods of preparing enzyme-antibody conjugates were evaluated. High yields of conjugate were obtained with both methods. The first procedure utilizes the homobifunctional crosslinking reagent N,N'-o-phenylenedimaleimide. Sulfydryl residues were introduced into second antibodies by reaction wit