A dihydrofolate reductase gene from Candida albicans: molecular cloning
β Scribed by M. Franceschi; M. Denaro; T. Irdani; R. Lorenzetti; G. Mastromei; E. Skarmoutsou; M. Polsinelli
- Book ID
- 109317103
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 210 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0378-1097
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Here we report the complete nucleotide sequence of a third chitinase gene ( C H T l ) from the dimorphic human pathogen Candida albicans. The deduced amino acid (aa) sequence of Chtl consists of 416 aa and displays 36% protein sequence similarity to chitinases Cht2 and Cht3, from C. albicans. Intere
The dihydrofolate reductase gene of Saccharornyces cerevisiae has been isolated by selection of trimethoprim resistant Escherichia coli transformed with a gene bank of yeast DNA in plasmid pBR322. From a 9.2 kilobase pair BamHI DNA fragment this gene has been localized to a 1.76 kb fragment, the res