A full-length cDNA clone for beta-isopropylmalate dehydrogenase from potato has been isolated and sequenced. The open reading frame is 1071 bp in length encoding a protein of 357 amino acids which includes a 29 amino acid, putative chloroplastic transit peptide. The amino acid sequence shows 33.3% a
Cloning and expression of the 3-isopropylmalate dehydrogenase gene from Candida albicans
β Scribed by Howard F. Jenkinson; Gillian P. Schep; Maxwell G. Shepherd
- Book ID
- 109314303
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 245 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0378-1097
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## 4 CONCLUSION We succeeded in expressing a fungal b-tubulin, which is contained in a very small amount in fungal cells and difficult to purify, in a large amount using the pET expression vector systems in E coli. The protein expressed using pET-32a(]) was puriΓΌed by nickel resin column chromatogr