A rapid spectrophotometric assay for cat
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Ronald T. Borchardt
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Article
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1974
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Elsevier Science
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English
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A new assay technique for catechol-0-methyltransferase is described. 3,4-Dihydroxyacetophenone is used as the substrate for the assay and the products, 3-hydroxy-4-methoxyacetophenone and 4-hydroxy-3-methoxyacetophenone are detected spectrophotometrically at 344 nm in borate buffer, pH 10.0. This sp