𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A device for the liberation and determination of 14CO2

✍ Scribed by Peter Schadewaldt; Malte E.C. Förster; Ulrich Münch; Wolfgang Staib

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
322 KB
Volume
132
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A simple closed system for the serial determination of 14CO2 in small volumes of fluid samples, is described. The device consists of commercially available scintillation vials and silicone tube seals. 14CO2 is selectively liberated by citric acid and absorbed in a scintillation vial by Hyamine. Experiments on the effect of dichloroacetate on pyruvate dehydrogenase activity in rat hindlimbs perfused with [1-14C]pyruvate demonstrate the applicability of the method.

📜 SIMILAR VOLUMES

Improved technique for accurate and conv
Improved technique for accurate and convenient assay of biological reactions liberating14CO2
✍ C.H. Sissons 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 417 KB

Use of a convenient and inexpensive apparatus for trapping 14CO2 from biological reactions in small volumes is described. Alternative scintillants for estimating "CO2 trapped in KOH are compared, toluene methoxyethanol (2:1) based scintillants and Instagel (TM-Packard) were efficient and more stable

A dialysis device for use in the determi
A dialysis device for use in the determination of density increments
✍ Peter J. Wells 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 112 KB

A procedure for the dialysis of small volumes of solution prior to density-increment determination is described. An easily constructed device, which can also be used for general dialysis procedures, facilitates solution transfer and ensures minimal exposure of the retentate to the atmosphere.

A Device for the Semiautomatic Determina
A Device for the Semiautomatic Determination of Oxygen-Radical Absorbance Capacity
✍ Charles R. Caldwell 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 208 KB

The oxygen-radical absorbance capacity (ORAC) assay has become a standard method to quantify the antioxidative properties of phytonutrients in fruit and vegetable extracts. However, it is usually not possible to determine directly the contribution of specific phytonutrients to the total ORAC value.