✦ LIBER ✦

Improved technique for accurate and convenient assay of biological reactions liberating14CO2

✍ Scribed by C.H. Sissons

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 417 KB
Volume: 70
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90470-x

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✦ Synopsis

Use of a convenient and inexpensive apparatus for trapping 14CO2 from biological reactions in small volumes is described. Alternative scintillants for estimating "CO2 trapped in KOH are compared, toluene methoxyethanol (2:1) based scintillants and Instagel (TM-Packard) were efficient and more stable than the dioxane/Cab-O-Sil or toluene/Triton X-100 mixtures usually used. The usefulness of the technique is illustrated with microassays of OM P decarboxylase (EC 4.1.1.23), OMP pyrophosphorylase (EC 2.4.2.10) in the fission yeast Schizosaccharomyces pombe.

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