A coupled assay for bile acid:CoA ligase
β Scribed by Donald A. Vessey; Andy Erdman; Michael Kelley; Tamara St. Claire
- Book ID
- 107718950
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 378 KB
- Volume
- 195
- Category
- Article
- ISSN
- 0003-2697
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Assays for endonuclease and ligase activities have been devised that make use of the fact that circular phage A DNA is preferentially retained on nitrocellulose filters while linear ADNA is not. The assay is rapid, time dependent, and quantitative and has been used for the purification of restrictio