An enzyme-coupled assay for glyoxylic acid
β Scribed by Sarah E Carpenter; David J Merkler
- Book ID
- 108047603
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 147 KB
- Volume
- 323
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
In 1937 Brunel (1) used the sensitive Rimini-Schryver reaction to identify glyoxylic acid as the product of the all,antoicase reaction. Young and Conway used this reaction for the determination of allantoin and allantoic acid (2). In their method, allantoin or allantoic acid is first hydrolyzed to g
A spectrophotometric assay for ureidoglycolase activity (both ureidoglycolate lyase and hydrolase), based on the reduction of glyoxylate to glycolate catalyzed by glyoxylate reductase or lactate dehydrogenase with the stoichiometric and continuous NADH oxidation, is described. The assay has been opt